Title:
One step multiplex aptamer selection and validation using magnetic nanoparticle aptamer library (aptaMAG) coupled to microfluidic surface plasmon resonance imaging biosensor
Agreement Value:
$24,972.00
Agreement Date:
Mar 23, 2020 - Mar 31, 2021
Description:
Aptamers have been proven as versatile molecular recognition elements, possessing specificity and selectivity comparable to that of antibodies, with additional unique properties: their synthesis is simpler, readily scalable, and less expensive; the can be designed to incorporate a variety of chemical modification; and they are much more stable than antibodies. The current gold standard of aptamer discovery is conducted through Systematic Evolution of Ligands by Exponential Enrichment (SELEX), which is very time and resource consuming, To remedy the shortcomings associated with this technique, the objective of this project is to develop a novel method for aptamer discovery. The team proposes to combine the oligonucleotide library design with the magnetic nanoparticles in concert with microfluidic surface plasmon resonance imaging biosensor capable of delivering one-step aptamer selection and validation technology. This proposed aptamer discovery method is both novel and has many advantages over the existing technologies. It significantly improves the ability to select high affinity and specificity aptamer akin to antibody counterparts and increases the applicability of the technology for the assays such as ELISA and immunoPCR.
Organization:
National Research Council Canada
Expected Results:
In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators.
Location:
Montreal, Quebec, CA H2X 0A8
Reference Number:
172-2019-2020-Q4-945032
Report Type:
Grants and Contributions
Recipient Business Number:
119128981
Additional Information:
This agreement has been amended 1 time(s). The end date of this agreements has been modified by 121 days. The total amended value is 30,000 dollars.
Recipient's Operating Name:
The Royal Institution for the Advancement of Learning/McGill University
Recipient's Legal Name:
The Royal Institution for the Advancement of Learning/McGill University
Federal Riding Name:
Ville-Marie–Le Sud-Ouest–Île-des-Sœurs
Federal Riding Number:
24077
Program:
Collaborative Science, Technology and Innovation Program – Ideation Fund
Program Purpose:
The Ideation Fund is intended to encourage, test and validate transformative research ideas generated by teams of NRC scientific personnel and external collaborators with complementary capabilities, acting as a demonstration phase for a continually-evolving suite of research and development (R&D) deliverables at the NRC. The fund supports exploratory research through two mechanisms: the New Beginnings Initiative and the Small Teams Initiative.