Grants and Contributions:
Title:
Genome-wide CRISPR screen to identify genes that increase the yield and functionality of AAV vectors.
Agreement Number:
947369
Agreement Value:
$200,000.00
Agreement Date:
Mar 20, 2020 - Mar 20, 2022
Description:
Adeno-associated virus production is typically carried out by transient transfection of 293 cells. It is therefore important to identify the molecular pathways in these cells that are critical for high-level viral production. This project will thus undertake a genome-wide screen for such host genes. The experimental strategy relies upon the up-regulation of cellular genes by CRISPR-dCasa and identification of those genes, whose overexpression results in an increase in AAV production. The long-term goal of this project would be to use this valuable information to genetically engineer 293SF-3F6 cells with the aim of generating a producer cell that has the ability to support high-level AAV production
Organization:
National Research Council Canada
Expected Results:
In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators. In the long term, find collaborative solutions to public policy challenges and create stronger innovation systems.
Location:
Montreal, Quebec, CA H3A 0G4
Reference Number:
172-2019-2020-Q4-947369
Agreement Type:
Grant
Report Type:
Grants and Contributions
Recipient Business Number:
121364749
Recipient Type:
Academia
Additional Information:
This agreement has been amended 1 time(s). The end date of this agreements has been modified by 61 days.
Recipient's Operating Name:
The Royal Institution for the Advancement of Learning/McGill University
Recipient's Legal Name:
The Royal Institution for the Advancement of Learning/McGill University
Federal Riding Name:
Ville-Marie–Le Sud-Ouest–Île-des-Sœurs
Federal Riding Number:
24077
Program:
Collaborative Science, Technology and Innovation Program - Collaborative R&D Initiatives
Program Purpose:
Collaborate on multiparty research and development programs to catalyze transformative, high-risk, high-reward research with the potential for game-changing scientific discoveries and technological breakthroughs in priority areas.
NAICS Code:
541710