Title:
Implementation of Single Cell Dielectrophoresis as a Tool for Bioprocess Optimization
Agreement Value:
$31,500.00
Agreement Date:
Mar 28, 2020 - Mar 31, 2022
Description:
Dielectric properties of cells play an important role during growth conditions in batch culture. Studies have demonstrated that the dielectric properties of cells can be used as an indicator of the state of cells in culture. Therefore, monitoring dielectric properties of cells such as intracellular conductivity during batch and fed-batch culture is being investigated as a tool for optimizing the productivity and quality of bioprocesses. Dielectric spectroscopy, known as capacitance measurement, is the key technique for monitoring the dielectric properties of the bulk cell culture. However, the bulk capacitance of a cell culture does not reveal information about the subpopulation of cells in the medium. The use of Dielectrophoresis (DEP) cytometer, which is a label-free, non-invasive and has the ability to be implemented as an online tool, for monitoring the dielectric properties of single-cells in batch and fed-batch culture identifies subpopulations. In this project, the DEP cytometer will be used to perform single-cell dielectric measurements under controlled conditions such as nutrient deprivation and timed feed addition scenarios to enhance the productivity and quality of bioprocesses.
Organization:
National Research Council Canada
Expected Results:
In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators.
Location:
Winnipeg, Manitoba, CA R3T 2N2
Reference Number:
172-2020-2021-Q4-946577
Report Type:
Grants and Contributions
Recipient Business Number:
119260669
Additional Information:
This agreement has been amended 1 time(s). The total amended value is $31500 and the end date of this agreements has been modified by 365 days)
Amendment Date
Jan 28, 2021
Recipient's Legal Name:
The University of Manitoba
Federal Riding Name:
Winnipeg South
Federal Riding Number:
46013
Program:
Collaborative Science, Technology and Innovation Program – Ideation Fund
Program Purpose:
The Ideation Fund is intended to encourage, test and validate transformative research ideas generated by teams of NRC scientific personnel and external collaborators with complementary capabilities, acting as a demonstration phase for a continually-evolving suite of research and development (R&D) deliverables at the NRC. The fund supports exploratory research through two mechanisms: the New Beginnings Initiative and the Small Teams Initiative.
NAICS Code:
541710 - R&D in the physical, engineering and life sciences