Grants and Contributions:
Title:
Developing of reagent combinations for the visual detection of SARS-CoV-2 - Phase 2
Agreement Number:
974543
Agreement Value:
$140,850.00
Agreement Date:
Jun 18, 2021 - Oct 29, 2021
Description:
The recipient proposes to optimize and perform a proof of concept study that combines microfluidic and molecular assay that detect SARS-CoV-2 from saliva by a color change in 30 minutes with minimum sensitivity of 10-18 mol. The microfluidic system will contain compartments with different steps of the assay. This assay consists of reagents that aim to amplify the SARS-CoV-2 genetic material (e.g. E gene) by 10 thousand fold. The amplified genetic material should bind to 13-nm gold nanoparticles coated with complementary single stranded DNA. The color of the solution should change because the gold nanoparticle will be assembled onto the SARS-CoV-2 amplified gene. The clustering of the gold nanoparticle changes how the electrons move leading to different absorbance properties. This physical process can be visualized by the change in the solution color from red to blue of the gold nanoparticles. A positive detection occurs when the solution is blue. The team propose to develop a microfluidic chip where different chambers to control each of the diagnostic assay reactions. The team will load diagnostic formulations (consist of enzymes, salts, and gold nanoparticle-conjugates) in each chamber. Individual reactions will occur by flowing the liquid from one chamber to the next in a consumable device that can be actuated with the use of an instrument in the simplest implementation.
Organization:
National Research Council Canada
Expected Results:
In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators. In the long term, find collaborative solutions to public policy challenges and create stronger innovation systems.
Location:
Toronto, Ontario, CA M5G 1L5
Reference Number:
172-2021-2022-Q1-974543
Agreement Type:
Grant
Report Type:
Grants and Contributions
Recipient Business Number:
108162330
Recipient Type:
Academia
Recipient's Legal Name:
The Governing Council of the University of Toronto
Federal Riding Name:
University–Rosedale
Federal Riding Number:
35110
Program:
Collaborative Science, Technology and Innovation Program - Collaborative R&D Initiatives
Program Purpose:
Collaborate on multiparty research and development programs to catalyze transformative, high-risk, high-reward research with the potential for game-changing scientific discoveries and technological breakthroughs in priority areas.
NAICS Code:
541710 - R&D in the physical, engineering and life sciences