Grants and Contributions:

Title:

Engineering, Cloning, Expression and Small Scale Purification of St1Cas9 and variants in Escherichia coli

Agreement Number:

956909

Agreement Value:

$100,000.00

Agreement Date:

Sep 17, 2020 - Sep 17, 2022

Description:

This Project seeks to produce recombinant St1Cas9 and variants and test their efficiency both in vitro and in vivo. These recombinant gene editing tools will become part of the toolkit the NRC-CGT Program aims to build to facilitate multiplex combinatorial gene editing in induced pluripotent stem cells (iPSCs) and immune cells. Along with the production of SpCas9, MAD7, and potentially Cas12a, the production of St1Cas9 and variants will broaden target flexibility and help to accelerate innovation and discovery cycles in engineered cell and gene therapies.

Organization:

National Research Council Canada

Expected Results:

In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators. In the long term, find collaborative solutions to public policy challenges and create stronger innovation systems.

Location:

Quebec, Quebec, CA G1V 0A6

Reference Number:

172-2020-2021-Q2-956909

Agreement Type:

Grant

Report Type:

Grants and Contributions

Recipient Business Number:

119278950

Recipient Type:

Academia

Recipient's Legal Name:

Université Laval

Federal Riding Name:

Louis-Hébert

Federal Riding Number:

24044

Program:

Collaborative Science, Technology and Innovation Program - Collaborative R&D Initiatives

Program Purpose:

Collaborate on multiparty research and development programs to catalyze transformative, high-risk, high-reward research with the potential for game-changing scientific discoveries and technological breakthroughs in priority areas.

NAICS Code:

541710