Grants and Contributions:
Title:
Optimizing the development of sgRNA array library: a PoC study
Agreement Number:
988599
Agreement Value:
$27,500.00
Agreement Date:
Mar 28, 2022 - Jul 31, 2024
Description:
The CRISPR/Cas system has revolutionized the analysis of gene function in mammalian cells. Cas cuts DNA at specific genomic region by associating with a single guide RNA (sgRNA) molecule whose sequence is complimentary to the genomic DNA to be targeted. This system was rapidly adopted by the functional genomic community to perform pooled screens because of its simplicity and efficiency. However, current pooled screen read-outs are limited to assays with strong selective ressure, but are ill fitted to measure phenotypic effect on state and differentiation of targeted cells. As such, arrayed screens are better suited for primary cells, such as iPSCs, neurons, and immune cells. Thus, the lack of a “whole-genome” arrayed sgRNA libraries, especially ribonucleoprotein (RNP) compatible, has impeded the functional characterization of multiple disease models targeted by the Cell and Gene Therapy (CGT) program. This proof of concept proof of concept (PoC) study seeks to develop a sgRNA arrayed library for use in cell types relevant to the CGT program.
Organization:
National Research Council Canada
Expected Results:
In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators. In the long term, find collaborative solutions to public policy challenges and create stronger innovation systems.
Location:
Toronto, Ontario, CA M5S 1A1
Reference Number:
172-2021-2022-Q1-988599
Agreement Type:
Grant
Report Type:
Grants and Contributions
Recipient Business Number:
108162330
Recipient Type:
Academia
Additional Information:
This agreement has been amended 3 time(s). The end date of this agreement has been modified by 212 days.
Amendment Date
Dec 22, 2023
Recipient's Legal Name:
The Hospital for Sick Children
Federal Riding Name:
University–Rosedale
Federal Riding Number:
35110
Program:
Collaborative Science, Technology and Innovation Program - Collaborative R&D Initiatives
Program Purpose:
Collaborate on multiparty research and development programs to catalyze transformative, high-risk, high-reward research with the potential for game-changing scientific discoveries and technological breakthroughs in priority areas.
NAICS Code:
541710
Amendments: