Grants and Contributions:

Title:

Digital droplet PCR assay development for single cell analytics

Agreement Number:

978650

Agreement Value:

$99,500.00

Agreement Date:

Sep 1, 2021 - Aug 31, 2022

Description:

This project aims to develop an analytical method to measure transduction efficiency at the single-cell level in a gene-modified, viral-vector based immunotherapy model. The method will employ digital droplet PCR (ddPCR) implemented in polymer microfluidic devices to encapsulate engineered cells with appropriate reagents (lysis and PCR master mix), including vector specific primers and hydrolysis probes, as well primers and probes for the reference gene. The encapsulated droplets will then be subjected to thermal cycling and subsequently arrayed in a monolayer format for fluorescence imaging. Image analysis and count of positive droplets for each fluorophore will allow accurate assessment of the transduction efficiency. This one-step lysis and digital PCR system aims at facilitating and streamlining the assessment of the viral transduction efficiency, an important step in the manufacturing of engineered T cell therapies.

Organization:

National Research Council Canada

Expected Results:

In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators. In the long term, find collaborative solutions to public policy challenges and create stronger innovation systems.

Location:

Hamilton, Ontario, CA L8S 4L8

Reference Number:

172-2021-2022-Q2-978650

Agreement Type:

Grant

Report Type:

Grants and Contributions

Recipient Business Number:

119035988

Recipient Type:

Academia

Recipient's Legal Name:

McMaster University

Federal Riding Name:

Hamilton West–Ancaster–Dundas

Federal Riding Number:

35038

Program:

Collaborative Science, Technology and Innovation Program - Collaborative R&D Initiatives

Program Purpose:

Collaborate on multiparty research and development programs to catalyze transformative, high-risk, high-reward research with the potential for game-changing scientific discoveries and technological breakthroughs in priority areas.

NAICS Code:

541710