Grants and Contributions:
Title:
Optimizing the development of sgRNA array library: a PoC study
Agreement Number:
988600
Agreement Value:
$27,500.00
Agreement Date:
Mar 28, 2022 - Mar 31, 2023
Description:
The CRISPR/Cas system has revolutionized the analysis of gene function in mammalian cells. Cas enzymes cut DNA at a specific genomic site as targeted by a single guide RNA (sgRNA) complimentary to the desired modification site. This system was rapidly adopted by the functional genomics community to perform pooled screens because of its simplicity and efficiency. However, current pooled screen read-outs are limited to assays with strong selective pressure, but are ill-suited to measure phenotypic effects on the state and differentiation of targeted cells. As such, arrayed screens are superior for primary cells, such as iPSCs, neurons, and immune cells. The lack of “whole-genome” arrayed sgRNA libraries, especially in ibonucleoprotein (RNP) formats that avoid introduction of foreign DNA elements, has impeded the functional characterization of multiple disease models targeted by the Cell and Gene Therapy (CGT) program. This proof of concept (PoC) study seeks to develop a sgRNA arrayed library for use in cell types relevant to the CGT program
Organization:
National Research Council Canada
Expected Results:
In the short term, anticipated outcomes will be strengthened collaborations across industry, academia, and government to support research excellence. In the medium term, anticipated outcomes will be the development of new and potentially disruptive technologies with collaborators. In the long term, find collaborative solutions to public policy challenges and create stronger innovation systems.
Location:
Montreal, Quebec, CA H3T 1P1
Reference Number:
172-2021-2022-Q4-988600
Agreement Type:
Grant
Report Type:
Grants and Contributions
Recipient Business Number:
108160995
Recipient Type:
Academia
Recipient's Legal Name:
University of Montreal
Federal Riding Name:
Outremont
Federal Riding Number:
24054
Program:
Collaborative Science, Technology and Innovation Program - Collaborative R&D Initiatives
Program Purpose:
Collaborate on multiparty research and development programs to catalyze transformative, high-risk, high-reward research with the potential for game-changing scientific discoveries and technological breakthroughs in priority areas.
NAICS Code:
541710